Cellular electron microscopy

  • 850 Pages
  • 4.69 MB
  • 7673 Downloads
  • English

Elsevier/Academic Press , Amsterdam, Boston
Electron microscopy, Microscopy -- Technique, Microscopy, Electron -- methods, Cell Physiology, Elektronenmicroscopie, Celbio
Statementedited by J. Richard McIntosh.
SeriesMethods in cell biology -- v. 79
ContributionsMcIntosh, J. Richard.
Classifications
LC ClassificationsQH212.E4 C45 2007
The Physical Object
Paginationxxvii, 850 p., [28] p. of plates :
ID Numbers
Open LibraryOL23734342M
ISBN 100123706475
ISBN 139780123706478
LC Control Number2008357277

Cellular Electron Microscopy will help biologists from many disciplines understand modern EM and the value it might bring to their own work. The book’s five sections deal with all major issues in EM of cells: specimen preparation, imaging in 3-D, imaging and understanding frozen-hydrated samples, labeling macromolecules, and analyzing EM : Hardcover.

Cellular Electron Microscopy will help biologists from many disciplines understand modern EM and the value it might bring to their own work.

The book’s five sections deal with all major issues in EM of cells: specimen preparation, imaging in 3-D, imaging and understanding frozen-hydrated samples, labeling macromolecules, and analyzing EM cturer: Academic Press. Using Electron Microscopy to Understand Functional Mechanisms of Chromosome Alignment on the Mitotic Spindle Bruce F.

McEwen, Yimin Dong, Kristin J. VandenBeldt Pages Cellular Electron Microscopy will help biologists from many disciplines understand modern EM and the value it might bring to their own work.

The book’s five sections deal with all major issues in EM of cells: specimen preparation, imaging in 3-D, imaging and understanding frozen-hydrated samples, labeling macromolecules, and analyzing EM data.

This book updates the numerous technological innovations that have improved the capabilities of electron microscopy.; It provides timely coverage of the subject given the significant rise in the number of biologists using light microscopy to answer their questions and the natural limitations of this kind of imaging.

ISBN: OCLC Number: Description: xxvii, pages, 28 unnumbered pages of plates: illustrations. Contents: The Physics of Rapid Cooling and Its Implications for Cryoimmobilization of Cells / J.

Dubochet --Cryopreparation Methods for Electron Microscopy of Selected Model Systems / K.

Download Cellular electron microscopy FB2

McDonald --Cryopreparation Methodology for. Each chapter discusses instrumentation and technology in relationship to application in research.

All of the common and cutting edge trends are covered - fluorescence / laser electron / nonlinear microscopy, infrared fluorescence, multiphoton imaging, tomography, FRAP, live imaging, STED, PALM/STORM, etc. Cellular Electron Microscopy will help biologists from many disciplines understand modern EM and the value it might bring to their own work.

The book's five sections deal with all major issues in EM of cells: specimen preparation, imaging in 3-D, imaging and understanding frozen-hydrated samples, labeling macromolecules, and analyzing EM data.

Get this from a library. Cellular Electron Microscopy. [J Richard McIntosh;] -- Recent advances in the imaging technique electron microscopy (EM) have improved the method, making it more reliable and rewarding, particularly in its description of three-dimensional detail.

This. image processing. Thus, the focus of the book is on the integrated microscope system, with foundations in optical theory but extensions into electronic imaging. Accordingly, the cover shows the conjugate field and aperture planes of the light microscope under the title “Fundamentals of Light Microscopy and Electronic Imaging.”.

Applications and Limitations in Scanning Electron Microscopy Visualization of Surface and Internal Cellular Structures. Interactions between Organisms.

Details Cellular electron microscopy PDF

Selective Detection of Surface Structures. Preparation Techniques The Transmission Electron Microscope. Cellular Electron Microscopy will help biologists from many disciplines understand modern EM and the value it might bring to their own work.

The book’s five sections deal with all major issues in EM of cells: specimen preparation, imaging in 3-D, imaging and understanding frozen-hydrated samples, labeling macromolecules, and analyzing EM : Elsevier Science.

Cambridge Core - Materials Science - Liquid Cell Electron Microscopy - edited by Frances M. Ross. As part of a microscopy service group, she is also connected to the wide array of other state-of-the-art microscopy techniques used in the field of plant research.

In recent years, Ulla has been particularly interested in 3D EM techniques and correlative light and electron microscopy approaches in the field of plant microscopy.

Browsing the scientific literature, electron microscopy was especially popular from the ’s to 80’s, whereas during the 90’s, with the advent of innovative molecular techniques, electron Author: Barry R.

Masters. This third edition of Electron Microscopy: Methods and Protocols expands upon the previous editions with current, detailed protocols on biological and molecular research techniques based on TEM and SEM as well as other closely related imaging and analytical new chapters on conventional and microwave assisted specimen, cryo-specimen preparation, negative.

This book highlights important techniques for cellular imaging and covers the basics and applications of electron tomography and related techniques. In addition, it considers practical aspects and broadens the technological focus by incorporating techniques that are only now becoming accessible (e.g.

block face imaging). A scanning electron microscope (SEM) is a type of electron microscope that produces images of a sample by scanning the surface with a focused beam of electrons interact with atoms in the sample, producing various signals that contain information about the surface topography and composition of the sample.

The electron beam is scanned in a raster scan. Key words: Cryo electron tomography, Cellular microscopy, Cryo specimen preparation, 3D recon- struction, Visualization During the last six decades, techniques to chemically fi.

Derived from the successful three-volume Handbook of Microscopy, this book provides a broad survey of the physical fundamentals and principles of all modern techniques of electron microscopy. This reference work on the method most often used for the characterization of surfaces offers a competent comparison of the feasibilities of the latest developments in this.

Electron Microscopy Methods and Protocols. Nasser Hajibagheri, editor"Methods in Molecular Biology", vspiral bound, ISBN See all chapters for this book. Electron Microscopy, 2nd Edition. John J. Bozzola & Lonnie D Russellpp, hardcover, ISBN Light Microscopy Can Distinguish Objects Separated by μm or More.

The compound microscope, the most common microscope in use today, contains several lenses that magnify the image of a specimen under study (Figure a).The total magnification is a product of the magnification of the individual lenses: if the objective lens magnifies fold (a X lens, the Cited by: 1.

Transmission electron microscopy (TEM, an abbreviation which can also stand for the instrument, a transmission electron microscope) is a microscopy technique in which a beam of electrons is transmitted through a specimen to form an image.

The specimen is most often an ultrathin section less than nm thick or a suspension on a grid. An image is formed from the interaction of. Electron Microscopy covers all of the important aspects of electron microscopy for biologists, including theory of scanning and transmission, specimen preparation, digital imaging and image analysis, laboratory safety and interpretation of images.

The 4/5(4).

Description Cellular electron microscopy FB2

Plant Cell Biology, volume in "Methods in Cell Biology", includes chapters on modern experimental procedures and applications developed for research in the broad area of plant cell biology.

Topics covered in this volume include techniques for imaging and analyzing membrane dynamics and movement across membranes; cell wall composition, structure and mechanics.

Much of this imaging has used isolated cellular subfractions that are small, stable, and symmetric enough to allow image averaging to facilitate high-resolution EM. The beam sensitivity of a frozen-hydrated sample requires that the total exposure to the electron beam be kept low (e.g., Cited by: A 5-day hands-on PhD training course in both Wageningen and Amsterdam.

After this course, organized jointly by Electron Microscopy Center Amsterdam and Wageningen Electron Microscopy Center, you will have insight into the possibilities of electron microscopy as a research tool and will be able to decide whether you should use scanning electron microscopy (SEM).

The go‐to resource for microscopists on biological applications of field emission gun scanning electron microscopy (FEGSEM). The evolution of scanning electron microscopy technologies and capability over the past few years has revolutionized the biological imaging capabilities of the microscope—giving it the capability to examine surface structures of cellular membranes to.

A beam of energetic electrons from an electron microscope interacts with the specimen in two main ways: 1) transmitted electrons, which are used for two-dimensional (2D) imaging in a transmission electron microscope (TEM) and 2) reflected electrons which are employed for 3D image formation in scanning electron microscopy (SEM).

Immunoelectron Microscopy: A Reliable Tool for the Analysis of Cellular Processes 67 General rules of thumb in immunogold electron microscopy - For immunoelectron microscopy, fixation is one of the most important steps in sample preparation due to the need to preserve as much biochemical reactivity as possible.

This volume demonstrates how cellular and associated electron microscopy contributes to knowledge about biological structural information, primarily at the nanometer level. It presents how EM approaches complement both conventional structural biology (at the high end, angstrom level of resolution) and digital light microscopy (at the low end, 1/5(1).

Figure \(\PageIndex{1}\): Light and Electron Microscopes: (a) Most light microscopes used in a college biology lab can magnify cells up to approximately times and have a resolution of about nanometers.

(b) Electron microscopes provide a much higher magnification, ,x, and a have a resolution of 50 picometers.The Simons Electron Microscopy Center provides expertise and resources for understanding both molecular and cellular structures. Molecular structure determination is enabled by high-end transmission electron microscopes (TEMs), direct detection cameras, and computational support for single particle analysis.